Chromotek gfp-trap agarose
WebGFP-PFKFB3 was overexpressed in HEK293T cells and immunoprecipitated using GFP-Trap agarose beads. The PFKFB3 complex was then incubated with the indicated amount of recombinant human Src protein for 10 min. Western blot was used to detect tyrosine-phosphorylation level. WebApr 11, 2024 · For example, we noted this caveat when purifying TDP-43 aggregates with a 434 Chromotek nanobody, GFP-trap magnetic agarose (#gta-10) instead of 10782-2-AP or 3H8. In this 435 scenario, we found that the nanobody was destabilized by the 1% Tween-20-containing extraction buffer 436 Jo urn al Pre- p oo f and resulted in virtually no yield …
Chromotek gfp-trap agarose
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WebIt natively comes with conventional UT, TOFD and all beam-forming phased array UT techniques for single-beam and multi-group inspection and its 3-encoded axis … WebProteintech gfp trap antibody conjugated agarose beads Gfp Trap Antibody Conjugated Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebJun 23, 2024 · Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines. PLoS ONE. 2016;11:e0154759 pubmed publisher … WebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Brand: Proteintech GTMA-400 Additional Details : Weight : 0.00480kg View more versions of this product Product Code. 17353363 55828.28 NOK / 400 reactions Quantity Estimated Shipment: 12-05-2024 Add to basket Description Specifications
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WebApr 11, 2024 · Sir2:eGFP was immunoprecipitated with ChromoTek GFP-Trap® Magnetic Agarose (ChromoTek, Planegg, Germany). Elution was performed by boiling the samples in LaemmLi buffer supplemented 200 mM DTT and proteins precipitated by addition of 4 volumes 1:1 methanol:acetone. Protein pellets were resuspended in urea 6 M, 50 mM …
WebProduct Information. GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. Description. … chronic rhinosinusitis in catsWebYou can use Chromotek GFP-trap magnetic agarose beads for the pull-down/co-immunoprecipitation assays. These magnetic beads are coupled to GFP nanobody and are ready to use. Only you need to label ... chronic rhinosinusitis prevalenceWebThe ChromoTek GFP-Trap is the gold standard for fast, reliable and effective one-step immunoprecipitation of GFP-fusion ... GFP-Trap Agarose u coupled to agarose beads 10 rxns (250µl resin) gta-10 20 rxns (500µl resin) gta-20 100 rxns (2.5ml resin) gta-100 GFP-TrapAgarose Kit derick lawsonWebChromoTek Host Alpaca Type Primary Clonality Monoclonal (unknown clone) Conjugate Agarose Target Green fluorescent protein (Water jellyfish) UniProt P42212 - GFP_AEQVI Gene GFP Modification Unmodified View Product On Supplier's Website Supplier provided information Immunogen GFP Validations None provided Applications derick longWebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ... chronic rhinosinusitis nice guidelinesWebDora D Robinson, age 70s, lives in Leavenworth, KS. View their profile including current address, phone number 913-682-XXXX, background check reports, and property record … chronic rhinosinusitis medicine managementWebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg … derick mcclain