Phic31 integrase

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August undefined, 2024

WebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ... WebMar 20, 2024 · The phiC31 integrase system, as a site-specific recombinase, could be a straightforward and efficient approach for this aim. According to the high potential of the application of phiC31 integrase system in stem cells, the present paper reviews previous studies, placing the focus on stem cell types and their application.

The therapeutic potential of phiC31 integrase as a gene therapy …

WebJun 19, 2014 · The ΦC31 system uses the properties of the site-specific integrase from the bacteriophage ΦC31, which mediates recombination between an attachment site ( attB) that is normally present in the... WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … criminal minds cinemorgue

Phage Integrases for Genome Editing SpringerLink

WebThe PhiC31 Integrase System Frequently used for gene therapy development and related applications Non-viral transgene delivery Single-step process Single-copy integration Preferential integration at active sites Delivery of inserts with no size constraints Easy generation of cell lines The PinPoint Targeted Integration System WebThe use of phiC31 integrase requires a defined sequence for insertion and is compatible with insertion of a single copy of the transgene. We describe the protocol we use to facilitate phiC31 integrase transgene insertion including the use of insulator sequences to reduce position effect disruption of transgene activity. WebMay 3, 2007 · ϕC31 integrase and several of its relatives are being widely used for precise engineering of complex genomes ( 1–8) and are emerging as promising new tools for gene therapy ( 9–15 ). In addition to being highly portable ϕC31 integrase is, unlike other recombinases used for genome manipulation such as Cre and Flp, unidirectional ( 7, 16, … mamino limited

Anti-Bacteriophage PhiC31 Integrase antibody (ab93248) - Abcam

Pinpoint & PhiC31 Integrase Systems System Biosciences

WebNational Center for Biotechnology Information WebMar 4, 2016 · Phage integrases are prokaryotic site-specific recombinases that perform precise cut-and-paste recombination between their short attB and attP recognition sequences. These enzymes work in cellular... mami nanami quotesWebpcDNA3.1 phiC31 was a gift from Konrad Basler (Addgene plasmid # 68310 ; http://n2t.net/addgene:68310 ; RRID:Addgene_68310) For your References section: An … mami mueve la cintura

"WebAug 11, 2011 · A generic PhiC31 integrase mediated exchange vector, pCB92, was assembled by modifying pExchange4-CB9 (derived from pRMCE [9]) with the insertion of a murine H19 insulator [20], obtained from pWHERE (Invivogen), together with a synthesized polylinker of unique sites to facilitate cloning. " - Phic31 integrase

Phic31 integrase

Using phage integrases in a site-specific dual integrase cassette ...

WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two … WebPhiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable. SBI’s PhiC31 …

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WebNov 1, 2013 · Because the phiC31 integrase gene coding sequence contains one NcoI restriction site, the purified fragments were first cloned into the plasmid pMD19-T simple (Takara, Dalian, China) to generate plasmid T-Int, then the 1.9-kb target DNA fragment was recovered by incomplete digestion with NcoI and XhoI from T-Int, and inserted into the … WebJan 1, 2024 · So far, only the phiC31 integrase has been shown to work efficiently in Parhyale (Kontarakis et al., 2011) and a version of the Raeppli clonal marker which is …

WebINTRODUCTION: The phiC31 integrase system is a phage-derived system that offers the ability to integrate plasmid DNA into the chromosomes at a subset of endogenous preferred locations associated with robust gene expression. Recent progress highlights the unique advantages of this system for in vivo gene therapy and for use in stem cells. WebPhiC31 integrase, a site-specific large serine recombinase, is a useful tool for genome engineering in a variety of eukaryotic species and cell types. PhiC31 integrase performs efficient recombination between its attB site and either its own placed attP site or a partially mismatched genomic pseudo attP site.

WebApr 1, 2010 · For systems using the φC31-integrase, the recognition sites on the PP are attB and attP sites (the bacterial and phage attachment sites of the φC31-integrase with approximately 40 bp each). WebJan 2, 2024 · The phiC31 integrase-mediated transgenesis system, which was established to clarify the biological functions of candidate genes associated with unique morphological, behavioral, and life-history traits in D. prolongata (Kudo et al. 2024b ), has several advantages, including high cargo capacity, transformation efficiency, and site-specific …

WebOver the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells.

WebFeb 22, 2024 · The introduced plasmids were 3.5 µg pBG2-V0b1-ins-ELuc-ins, 7 µg GLV2-tdTomato, 10.5 µg GLV3-NeoR-BFP, 3 µg pEF1a-Cre, 3 µg pCAG-Bxb1 integrase, and 3 µg … criminal minds ciaWebJan 14, 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site ( attB) and a phage attachment site ( attP ). mami monchita\u0027s menuWebThe phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also mediates integration … criminal minds dale schraderWebThe phiC31 integrase system has, for the first time, enabled transformation of flies with BAC-sized constructs . The integrase can be expressed from mRNA co-injected with the construct or from the genome under a germ-line specific promoter, the latter method being more efficient . Several dozens of ... criminal minds commercial songWebOct 2, 2024 · One possibility is to use the integrase of phage phiC31 (phiC31-int) which catalyzes efficient site-specific recombination … ma minimum auto insurance limitsWebVector and parameters for targeted transgenic RNA interference in Drosophila melanogaster Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians mamino deliveryWebFinally, the serine integrase PhiC31 was also applied in the yeast Saccharomyces cerevisiae as a marker recovery tool, indicating the … criminal minds common sense media