Webb快速连接步骤： 1、取50 ng载体和3倍摩尔量的插入片段，用双蒸水调整总体积为10μl。 2、加入10μl 2×Quick Ligation Buffer，混匀。 3、加入0.5～1μl T4 DNA连接酶，充分但轻柔混匀（切勿剧烈震荡，可能导致酶部分失活）。 4、稍事离心，置于室温 (25℃) 作用5分钟。 5、冰上放置，转化（如不立即进行转化实验请冻 存于-20℃） 相关搜索： T4连接 … Webb产品简介 T4 DNA Ligase 在 ATP 作为辅助的情况下，可以特异性催化双链 DNA 或者双链 RNA 的 5 ’磷酸和 3 ’羟基之间形成磷酸二酯键。 本产品对与粘性末端和平末端的双链核酸 …
T4 DNA Ligase (1 U/µL) - Thermo Fisher Scientific
Webb14 apr. 2024 · The DNA was then pulled down with 75 μL Dynabeads M-280 Streptavidin (Thermo Fisher Scientific, Waltham, MA, USA). ... China). The products were ligated with T4 DNA ligase (TaKaRa, Dalian, Liaoning, China) at 16 °C o/n. The recombinant plasmids were confirmed by Sanger sequencing (Sangon Biotech, Shanghai, China) and co ... WebbT4 DNA连接酶常用于催化双链DNA平末端或互补粘性末 端之间的连接反应，也能催化双链RNA 5'-磷酸末端和3'-羟基 末端间的连接。 还可以修复双链DNA、RNA或DNA/RNA杂 交双 … kitchen cabinet island diy
TransformAid Bacterial Transformation Kit - Thermo Fisher Scientific
Webb15 maj 2024 · ATP-binding cassette (ABC) transporters are a superfamily of proteins that transport nutrient substances and secondary metabolites through cell membranes. They also act as an uptake system for N,N′-diacetylchitobiose (GlcNAc)2 in Streptomyces coelicolor. (GlcNAc)2 is an important inducer of chitinase. However, whether the ABC … Webb14 apr. 2024 · IntroductionThe northern Red Sea has been coined a refuge for reef corals due to the exceptional thermal tolerance of these organisms. With ocean warming threatening coral reefs worldwide, a panoptic characterization of corals living in extreme conditions may provide insights into future responses of corals to environmental … kitchen cabinet is pulling away from wall